Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter February 23, 2021

Critical role of pre-analytical aspects for the measurement of circulating calprotectin in serum or plasma as a biomarker for neutrophil-related inflammation

  • Maria Infantino EMAIL logo , Mariangela Manfredi , Roger Albesa , Valentina Grossi , Barbara Lari , Maurizio Benucci , Francesca Li Gobbi , Alessandra Matucci , Filippo Sarra and Michael Mahler

Corresponding author: Dr. Maria Infantino, Immunology and Allergology Laboratory Unit, S. Giovanni di Dio Hospital, Via Torregalli, 3 50143Florence, Italy, Phone: +39 0556932502; Fax: +39 0556932289, E-mail:

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Ethical approval: The study was conducted in accordance with the Declaration of Helsinki and the Italian legislation (Authorization of the Privacy Guarantor n. 9, 12 December 2013).

References

1. Ometto, F, Friso, L, Astorri, D, Botsios, C, Raffeiner, B, Punzi, L, et al.. Calprotectin in rheumatic diseases. Exp Biol Med 2017;242:859–73. https://doi.org/10.1177/1535370216681551.Search in Google Scholar PubMed PubMed Central

2. Wang, Q, Chen, W, Lin, J. The role of calprotectin in rheumatoid arthritis. J Transl Int Med 2019;7:126–31. https://doi.org/10.2478/jtim-2019-0026.Search in Google Scholar PubMed PubMed Central

3. Dale, I. Plasma levels of the calcium-binding L1 leukocyte protein: standardization of blood collection and evaluation of reference intervals in healthy controls. Scand J Clin Lab Invest 1990;50:837–41. https://doi.org/10.3109/00365519009104950.Search in Google Scholar PubMed

4. Van Hoovels, L, Vander Cruyssen, B, Bogaert, L, Van den Bremt, S, Bossuyt, X. Pre-analytical and analytical confounders of serum calprotectin as a biomarker in rheumatoid arthritis. Clin Chem Lab Med 2019;18:40–9. https://doi.org/10.1515/cclm-2019-0508.Search in Google Scholar PubMed

5. Rammes, A, Roth, J, Goebeler, M, Klempt, M, Hartmann, M, Sorg, C. Myeloid-related protein (MRP) 8 and MRP14 calcium-binding proteins of the S100 family are secreted by activated monocytes via a novel tubulin-dependent pathway. J Biol Chem 1997;272:9496–502. https://doi.org/10.1074/jbc.272.14.9496.Search in Google Scholar PubMed

6. Clark, SR, Ma, AC, Tavener, SA, McDonald, B, Goodarzi, Z, Kelly, MM, et al.. Platelet TLR4 activates neutrophil extracellular traps to ensnare bacteria in septic blood. Nat Med 2007;13:463–9. https://doi.org/10.1038/nm1565.Search in Google Scholar PubMed

7. Urban, CF, Ermert, D, Schmid, M, Abu-Abed, U, Goosmann, C, Nacken, W, et al.. Neutrophil extracellular traps contain calprotectin, a cytosolic protein complex involved in host defense against Candida albicans. PLoS Pathog 2009;5:e1000639. https://doi.org/10.1371/journal.ppat.1000639.Search in Google Scholar PubMed PubMed Central

8. Steinberg, BE, Grinstein, S. Unconventional roles of the NADPH oxidase: signaling, ion homeostasis, and cell death. Sci STKE 2007;2007:pe11. https://doi.org/10.1126/stke.3792007pe11.Search in Google Scholar PubMed

9. Pedersen, L, Birkemose, E, Gils, C, Safi, S, Nybo, M. Sample type and storage conditions affect calprotectin measurements in blood. J Appl Lab Med 2018;2:851–6. https://doi.org/10.1373/jalm.2017.024778.Search in Google Scholar PubMed

10. Åsberg, A, Løfblad, L, Felic, A, Hov, GG. Measuring calprotectin in plasma and blood with a fully automated turbidimetric assay. Scand J Clin Lab Invest 2019;79:50–7. https://doi.org/10.1080/00365513.2018.1550810.Search in Google Scholar PubMed

11. Nordal, HH, Magne, K, Fagerhol, MK, Halse, AK, Hammer, HB. Calprotectin (S100A8/A9) should preferably be measured in EDTA-plasma: results from a longitudinal study of patients with rheumatoid arthritis. Scand J Clin Lab Invest 2018;78:102–8. https://doi.org/10.1080/00365513.2017.1419371.Search in Google Scholar PubMed

12. Stephan JR, YF, Costello, RM, Bleier, BS, Nolan, EM. Oxidative post-translational modifications accelerate proteolytic degradation of calprotectin. J Am Chem Soc 2018;140:17444–55. https://doi.org/10.1021/jacs.8b06354.Search in Google Scholar PubMed PubMed Central

Received: 2020-09-02
Accepted: 2021-02-11
Published Online: 2021-02-23
Published in Print: 2021-07-27

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Downloaded on 24.4.2024 from https://www.degruyter.com/document/doi/10.1515/cclm-2021-0172/html
Scroll to top button