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Perinatal asphyxia partly affects presepsin urine levels in non-infected term infants

  • Valentina Botondi , Alice Pirra , Mariachiara Strozzi , Marika Perrotta , Danilo A.W. Gavilanes , Laura Di Ricco , Cynzia Spagnuolo , Antonio Maconi , Andrea Rocchetti , Laura Mazzucco , Valeria Balbo , Federico Schena , Giuseppina Stellitano , Arianna Oddi , Andrea Dotta , Iliana Bersani , Andrea Sannia , Chiara Peila , Enrico Bertino , Ines Bianco , Alessandra Gambi , Rocco Mangifesta and Diego Gazzolo EMAIL logo
Published/Copyright: February 4, 2022
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 60 Issue 5

Abstract

Objectives

Standard of care sepsis biomarkers such as C-reactive protein (CRP) and procalcitonin (PCT) can be affected by several perinatal factors, among which perinatal asphyxia (PA) has a significant role. In this light, new early sepsis biomarkers such as presepsin (P-SEP) are needed to enact therapeutic strategies at a stage when clinical and laboratory patterns are still silent or unavailable. We aimed at investigating the potential effects of PA on longitudinal P-SEP urine levels.

Methods

We conducted an observational case-control study in 76 term infants, 38 with PA and 38 controls. Standard clinical, laboratory, radiological monitoring procedures and P-SEP urine measurement were performed at four time-points (first void, 24, 48, 96 h) after birth.

Results

Higher (p<0.05) CRP and PCT blood levels at T1–T3 were observed in PA than control infants whilst no differences (p>0.05, for all) at T0 were observed between groups. P-SEP urine levels were higher (p<0.05) in PA at first void and at 24 h while no differences (p>0.05) at 48 and 96 h were observed. No significant correlations were found (p>0.05) between P-SEP and urea (R=0.11) and creatinine (R=0.02) blood levels, respectively.

Conclusions

The present results, showed that PA effects on P-SEP were limited up to the first 24 h following birth in absence of any kidney function bias. Data open the way to further investigations aimed at validating P-SEP assessment in non-invasive biological fluids as a reliable tool for early EOS and LOS detection in high-risk infants.

Keywords: kidney; newborn; perinatal asphyxia (PA); presepsin (P-SEP); sepsis
Corresponding author: Prof. Diego Gazzolo, Neonatal Intensive Care Unit, G. d’Annunzio University, 65100 Chieti, Italy, Phone: +39 0871 358219, E-mail:

Funding source: I Colori della Vita Foundation

Award Identifier / Grant number: Grant 2019/3 IO PhD International

Funding source: Mellin, Danone-Nutricia Benefit

Award Identifier / Grant number: Presap ASL-NL-6229

Acknowledgments

We thank Gepa s.r.l., Italy, for supporting analysis kits.

  1. Research funding: This work is part of the I.O. PhD International Program under the auspices of the Italian Society of Neonatology and was partially supported by grants to DG from “I Colori della Vita Foundation (Grant 2019/3 IO PhD International),” Italy, and by unconditioned sponsorship to DG from Mellin, Danone-Nutricia Benefit (Presap ASL-NL-6229), Italy.

  2. Author contributions: Valentina Botondi, Alice Pirra, Chiara Strozzi, Marika Perrotta, Danilo AW Gavilanes, Laura Di Ricco, Cynzia Spagnuolo, Antonio Maconi, Andrea Rocchetti, Laura Mazzucco, Valeria Balbo, Federico Schena, A Giuseppina Stellitano, Arianna Oddi, Andrea Dotta, Iliana Bersani, Andrea Sannia, Chiara Peila, Enrico Bertino, Ines Bianco, Alessandra Gambi, Rocco Mangifesta and Diego Gazzolo made substantial contribution to conception and design of the study, acquisition, analysis, and interpretation of data; authors participate in drafting the article or revising it critically. All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: The parents of the subjects admitted in the study gave informed and signed consent.

  5. Ethical approval: Research involving human subjects complied with all relevant national regulations, institutional policies and is in accordance with the tenets of the Helsinki Declaration (as revised in 2013). The local Ethic Committees of the Institutions approved the study protocol (Presap.ASO.Neonat.19.02/ 23.05.19).

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Received: 2021-08-19
Accepted: 2022-01-14
Published Online: 2022-02-04
Published in Print: 2022-04-26

© 2022 Walter de Gruyter GmbH, Berlin/Boston

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DOI:  doi.org/10.1515/cclm-2022-0027
Keywords for this article
kidney; newborn; perinatal asphyxia (PA); presepsin (P-SEP); sepsis

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. Lot-to-lot variation: no longer a neglected issue
  4. Review
  5. Health Technology Assessment to assess value of biomarkers in the decision-making process
  6. Mini Review
  7. Diagnostic performance of the fully automated Roche Elecsys SARS-CoV-2 antigen electrochemiluminescence immunoassay: a pooled analysis
  8. Opinion Papers
  9. Preanalytical quality improvement – an interdisciplinary journey
  10. Metrological traceability and clinical traceability of laboratory results – the role of commutability in External Quality Assurance
  11. Lot-to-lot reagent verification: challenges and possible solutions
  12. EFLM Paper
  13. An approach for determining allowable between reagent lot variation
  14. General Clinical Chemistry and Laboratory Medicine
  15. Introduction of BD Vacutainer® Barricor™ tubes in clinical biobanking and application of amino acid and cytokine quality indicators to Barricor plasma
  16. Delays during PBMC isolation have a moderate effect on yield, but severly compromise cell viability
  17. Investigation of the effects of pneumatic tube transport system on routine biochemistry, hematology, and coagulation tests in Ankara City Hospital
  18. Comparison of three different protocols for obtaining hemolysis
  19. Report from the HarmoSter study: impact of calibration on comparability of LC-MS/MS measurement of circulating cortisol, 17OH-progesterone and aldosterone
  20. Point-of-care testing in primary healthcare: a scoring system to determine the frequency of performing internal quality control
  21. Hematology and Coagulation
  22. The flagging features of the Sysmex XN-10 analyser for detecting platelet clumps and the impacts of platelet clumps on complete blood count parameters
  23. Cancer Diagnostics
  24. Thyroglobulin and thyroglobulin antibodies: assay-dependent management consequences in patients with differentiated thyroid carcinoma
  25. Infectious Diseases
  26. Hyris bCUBE SARS-CoV-2 rapid molecular saliva testing: a POCT innovation on its way
  27. Ultrasensitive assay for saliva-based SARS-CoV-2 antigen detection
  28. Identification of contagious SARS-CoV-2 infected individuals by Roche’s Rapid Antigen Test
  29. Monocyte distribution width (MDW) as a screening tool for early detecting sepsis: a systematic review and meta-analysis
  30. Perinatal asphyxia partly affects presepsin urine levels in non-infected term infants
  31. Letters to the Editors
  32. Spurious results for total and free prostate-specific antigen (PSA); sometimes really “a riddle wrapped in a mystery inside an enigma”
  33. Reply to: Spurious results for total and free prostate-specific antigen (PSA); sometimes really “a riddle wrapped in a mystery inside an enigma”
  34. Spike vs. nucleocapsid serum antigens for COVID-19 diagnosis and severity assessment
  35. A rapid semi-quantitative test for determination of SARS-CoV-2 antibody levels
  36. Diagnostic potential of leukocyte differential and cell population data in prediction of COVID-19 among related viral and bacterial infections at Emergency Department
  37. Added value of drug-laboratory test interaction alerts in test result authorisation
  38. Pre-analytical considerations for the analysis of uracil and 5,6-dihydrouracil in heparin plasma
  39. Rare unstable and low oxygen affinity haemoglobin variant, Hb Hazebrouck, detected on Sysmex XN-9000
  40. Multicancer early detection
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